Abstract

Background and objectives: Research on identification of some common Staphyloccoci species on healthy human skin at the University of Medicine and Pharmacy, Hue University with the aim of using biological methods to biologically identify and survey the distribution of S. aureus, S. epidermidis and S. saprophyticus on healthy human skin. Subjects and methods: Cross-sectional descriptive study of the results of isolation by routine bacterial culture at the Department of Microbiology, Hue University Hospital. Results: Identification of staphylococci on healthy human skin by biological methods on 249 staphylococci strains, of which S. aureus had 11 strains (4.4%), S. epidermidis had 128 strains accounting for 51.4%, S. saprophyticus had 74 strains accounting for 36.6% and other CoNS had 36 strains accounting for 14.5%. The biological identification method with Gram staining, catalase, coagulase, mannitol fermentation, hemolysis and novobiocin susceptibility testing for the diagnosis of staphylococci, in particularly, the two important tests, coagulase and novobiocin resistance are the most important to identify S. aureus, S. epidermidis and S. saprophyticus which does not require high equipment requirements, is not technically demanding, and can give results quite quickly (possibly within 36 - 48 hours). Conclusion: Biological methods can be used to identify some staphylococci species to meet the needs of microbiological diagnosis at the district level