Tóm tắt
Introduction: Teeth displaced from their alveolar bone can be replanted if properly handled and stored in suitable environments. To help minimize the failure of reimplantation, teeth should be preserved in a storage environment that is initially free of bacteria.
Research objective: Evaluating the morphology of bacteria on the periodontal ligament cells in different storage environments at various time points after the teeth have been removed from the alveolar bone using Gram staining.
Materials and Methods: An in vitro study without a control group was conducted using 48 premolars and third molars after extraction, which were stored in four environments: Dulbecco’s modified Eagle’s medium (DMEM), fresh whole milk (unsweetened), saline solution, and electrolyte-replenishing drink. The presence of bacteria was assessed at four time points: 30 minutes, 1 hour, 2 hours, and 24 hours. Results: In DMEM and fresh whole milk, Gram-positive cocci, Gram-positive bacilli, and Gram-negative bacilli were presented at 30 minutes, 1 hour, and 2 hours; by 24 hours, Gram-negative cocci were also presented. In the saline solution, Gram-positive cocci and bacilli were also presented at all time points; Gram-negative bacilli appeared at 2 and 24 hours. In the electrolyte-replenishing drink, Gram-positive cocci were the only bacteria found at all time points.
Conclusion: Bacteria were presented on the periodontal ligament cells of the root surface in all four environments and at all four-time points of storage. However, at different time points, the number of bacterial groups found in DMEM and fresh whole milk was the highest and nearly equivalent, followed by the saline solution and the lowest count in the electrolyte-replenishing drink.
| Đã xuất bản | 09-05-2025 | |
| Toàn văn |
|
|
| Ngôn ngữ |
|
|
| Số tạp chí | Tập 15 Số 2 (2025) | |
| Phân mục | Nghiên cứu | |
| DOI | 10.34071/jmp.2025.2.11 | |
| Từ khóa | bacteria, periodontal ligament cells, alveolar bone |
công trình này được cấp phép theo Creative Commons Attribution-phi thương mại-NoDerivatives 4.0 License International .
Bản quyền (c) 2025 Tạp chí Y Dược Huế
Glendor U., Halling A., Andersson L., et al. Incidence of traumatic tooth injuries in children and adolescents in the county of Vastmanland, Sweden. Swed Dent J. 1996;20(1-2):15-28.
Levin L., Day P.F., Hicks L. et al. International Association of Dental Traumatology guidelines for managing traumatic dental injuries: General introduction. Dent Traumatol. 2020;36(4):309-13.
De Brier N., Borra V., et al. Storage of an avulsed tooth before replantation: A systematic review and meta-analysis. Dent Traumatol. 2020;36(5):453-76.
Martin M.P., Pileggi R. A quantitative analysis of Propolis: a promising new storage media following avulsion. Dent Traumatol. 2004;20(2):85-89.
Bijlani S., Shanbhog R., Godhi B.S. et al. An in vitro evaluation of ice apple water, Aloe vera, and propolis as a storage medium to preserve viability of human periodontal ligament fibroblasts. J Indian Soc Pedod Prev Dent. 2022;40(2):195-200.
Poi W.R., Sonoda C.K., Martins C.M. et al. Storage media for avulsed teeth: a literature review. Braz Dent J. 2013;24(5):437-45.
Chamorro M.M., Regan J.D., Opperman L. A. et al. Effect of storage media on human periodontal ligament cell apoptosis. Dent Traumatol. 2008;24(1):11-16.
Dhimole P., Bhayya D.P., Gupta S. et al. Evaluation of the efficacy of neem (Azadirachta indica) and turmeric (Curcuma longa) as storage media in maintaining periodontal ligament cell viability: An in vitro study. J Indian Soc Pedod Prev Dent. 2019;37(2):140-45.
Hasanuddin S., Reddy J.S. Sequelae of delayed replantation of maxillary permanent incisors after avulsion: A case series with 24-month follow-up and clinical review. J Indian Soc Pedod Prev Dent. 2018;36(4):410-16.
Poi W.R., Sonoda C.K., Martins C.M. et al. Storage media for avulsed teeth: a literature review. Braz Dent J. 2013;24(5):437-45.
Tuna E.B., Yaman D. and Yamamato S. What is the Best Root Surface Treatment for Avulsed Teeth?. Open Dent J. 2014;8:175-79.
Moyes R.B., Reynolds J. and Breakwell D.P. Differential staining of bacteria: gram stain. Curr Protoc Microbiol. 2009;15(1):1-8.
Thairu Y., Nasir I.A. and Usman Y. Laboratory perspective of gram staining and its significance in investigations of infectious diseases. Saharan Afr J Med. 2014;1(4):168-74.
Iwasaki K., Akazawa K., Nagata M. et al. Angiogenic Effects of Secreted Factors from Periodontal Ligament Stem Cells. Dent J (Basel). 2021;9(1):2-11.
Fouad A.F. Microbiological Aspects of Traumatic Injuries. J Endod. 2019;45(12):39-48.
Caglar E., Sandalli N., Kuscu O. et al. Viability of fibroblasts in a novel probiotic storage media. Dent Traumatol. 2010;26(5):383-387.
Fouad A.F., Abbott P.V., Tsilingaridis G. et al. International Association of Dental Traumatology guidelines for the management of traumatic dental injuries: 2. Avulsion of permanent teeth. Dent Traumatol. 2020;36(4):331-42.