Indexing
CÁC SỐ TỪ 2011-2023
Tạp chí Y Dược Học
Abstract
Background: 10-deacetylbaccatin III (10-DAB III) extracted from Taxus species is an important precursor for the semi-synthesis of the anticancer drugs paclitaxel and docetaxel.
Objectives: Therefore, this study was conducted to develop an HPLC method for the quantification of 10-DAB III in Taxus wallichiana Zucc. samples.
Materials and Methods: Leaf samples of T. wallichiana were collected in Lam Dong province and extracted with methanol-water (9:1, v/v). Suitable chromatographic conditions were selected, and the method was validated in accordance with AOAC guidelines.
Results: Chromatographic separation was achieved on a C18 column using a mobile phase consisting of acetonitrile and 0.1% aqueous trifluoroacetic acid (30:70, v/v). The HPLC method demonstrated specificity, with relative standard deviations (RSDs) below 2.1% and recoveries ranging from 98,63 to 103,86%. The 10-DAB III content in the two leaf samples ranged from 2.58 to 2.72 mg/g on a dry-weight basis.
Conclusion: The developed HPLC-PDA method met the evaluated validation criteria and was suitable for quantifying 10-DAB III in T. wallichiana leaves.
| Published | 2026-10-14 | |
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| Issue | Vol. 17 No. ONLINE FIRST (2027) | |
| Section | Original Articles | |
| DOI | 10.34071/jmp.2027.1.1631 | |
| Keywords | 10-Deacetylbaccatin III (10-DAB III), Thông đỏ, HPLC-PDA. |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2026 Hue Journal of Medicine and Pharmacy
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