Abstract

Background: Currently, the by-products after processing Basa fish are quite large, and this is also a source of raw materials with many nutritional components. Research and develop a process to extract fish oil from Basa fish by-products by green extraction technique with DES solvent system and analyze the omega-3, 6, and 9 content of the obtained fish oil in order to take advantage of this excess material. Materials and methods: Basa fish by-products, including fat, skin, head, and viscera were processed and defatted. Separation of fish oil from fat obtained by liquid-liquid extraction with alcohol and DES solvent systems was choline chloride:urea (1:1). Fish oil after separation was processed, purified, and analyzed for chemical composition by a GC-MS system. Results: Fish oil containing 84.5% Omega-3, 6, and 9 was isolated from Basa fish by-products, in which EPA and DHA content were 40.5 and 18.9%, respectively. The separation efficiency of fish oil from the raw material and from the fat layer were 5.4 and 25.0%, respectively. Conclusions: The process of extracting fish oil containing Omega-3, 6, and 9 from Basa fish by-products is quite simple, cheap, and safe, with high efficiency, promising a lot of potential for large-scale upgrading to be applied in production practice