Abstract

Objectives: the study aimed to investigate the antibiotic resistance profile and to determine the preva­lence of carbapenem resistance genes in Klebsiella pneumoniae strains isolated from the patients hospital­ized in Hue Central Hospital and Hue University of Medicine and Pharmacy Hospital. Methods: traditional microbiological methods were used for bacterial identification, antibiotic susceptible testing and phenotypic method for detection of extended spectrum beta-lactamases on 145 Klebsiella pneumoniae strains isolated from the clinical samples. Multiplex PCR was used for detection of blaVIM and blaIMP genes. Results: Klebsiella pneumoniae strains showed high rates of resistance to several antibiotics such as cefuroxime (54.2%), cef­triaxone (74.5%), cefepime (66.5%), meropenem (53.7%), ertapenem (57.9%) and ciprofloxacin (78.8%). The rate of Klebsiella pneumoniae isolates with multidrug resistance (MDR) was 65.5% and resistant rate to 5 or more antibiotics was found at 63.4% of bacterial isolates. The rate of ESBL-producing Klebsiella pneumoniae was 42.8%, ESBL-producing strains showed a higher rate of antibiotic resistance than non-ESBL-producing strains. The prevalence of blaVIM and blaIMP genes of Klebsiella pneumoniae isolates was 22.1% and 11.7%, respectively, of which 6 strains (4.1%) carried both genes. Conclusions: Klebsiella pneumoniae isolates were highly resistant to several antibiotics, the rate of MDR was 65.5%, the rate of ESBL enzyme production was 42.8%. The prevalence of blaVIM and blaIMP genes was 22.1% and 11.7% respectively.